The Proteomics Playbook

Analysis of proteins is not simple. Unlike DNA, there is incredible diversity in the concentration and variety of proteins created by a cell at any given moment.  

In our Proteomics Playbook we outline the principles, methods and available technologies to help you overcome common hurdles faced by the proteomics field.  

This report will help you explore the basic principles of sample preparation, mass spectrometry technologies and protein sequencing, spatial proteomics, and AI models for protein structure prediction and design. We highlight commercial options for biomarker discovery and present the most impactful clinical proteomics research this year had to offer. 

Throughout this report, we include unique insights from our contributors to provide hints and tips to apply to your own research. We hope that this inspires you to tackle proteomics in your own work and help drive advancements in this exciting field! 

We hear from:  

• Wout Bittremieux, Assistant Research Professor, University of Antwerp 

• Anton Calabrese, Sir Henry Dale Fellow and University Academic Fellow Group Leader, University of Leeds 

• Rachael Cartwright, Senior Project Manager, Medical Research Council (UK Research and Innovation) 

• Zhengming Chen, Richard Peto Professor of Epidemiology, Nuffield Department of Population Health, University of Oxford 

• Dr Maarten Dhaenens, Postdoctoral Researcher and Proteomics Core Team Leader, Ghent University 

• Fredrik Edfors, Assistant Professor, DDLS SciLifeLab 

• Rohith Krishna, Graduate Student, University of Washington 

• Adam Lewandowski, Associate Professor, University of Oxford; Deputy Chief Scientist, UK Biobank 

• Stacy Malaker, Assistant Professor of Chemistry, Yale University 

• Evan Mills, Vice President, Sales America, Olink Proteomics 

• Andreas Mund, Associate Professor, Novo Nordisk Foundation Center for Protein Research; Co-Founder and CSO, OmicVision Biosciences (BioInnovation Institute, Ole Maaløes Vej 3, 2200 København, Denmark) 

• Lucas Nivon, Co-founder, OpenFold 

• Keren Papier, Senior Nutritional Epidemiologist, University of Oxford 

• Ana Robles, Program Director, Office of Cancer Clinical Proteomics Research, NCI 

• Karin Rodland, Director Precision Medicine Innovation CoLaboratory, Pacific Northwest National Laboratory 

• Hanno Steen, Associate Professor of Pathology, Harvard Medical School 

• Benjamin Sun, Director, BMS 

• Mallory Tollefson, Business Development Manager, OpenFold Consortium 

• Mathias Uhlén, Professor of Microbiology, Science for Life Laboratory and Albanova University Center, Royal Institute of Technology (KTH) 

• Stephanie Wankowicz, Assistant Professional Researcher, University of California San Francisco